2 mL (1 mL min.) serum from 1 Red Top (No Additive) tube. Do not use gel barrier tubes. Wrap in Aluminium foil to protect from light. Ship refrigerated or frozen.
Sample by Tue, Thu, Sat 1 pm
Report 2nd day Evening
Blood concentrations of Methotrexate are monitored at 24, 48 & 72 hours after a single high dose (>50 mg/m2) therapy.